A Simulation Model of the Planetary Boundary Layer at Kennedy Space Center

A simulation model which predicts the behavior of the Atmospheric Boundary Layer has been developed and coded. The model is partially evaluated by comparing it with laboratory measurements and the sounding measurements at Kennedy Space Center. The applicability of such an approach should prove quite widespread

Isotrivial VMRT-structures of complete intersection type

The family of varieties of minimal rational tangents on a quasi-homogeneous projective manifold is isotrivial. Conversely, are projective manifolds with isotrivial varieties of minimal rational tangents quasi-homogenous? We will show that this is not true in general, even when the projective manifold has Picard number 1. In fact, an isotrivial family of varieties of minimal rational tangents needs not be locally flat in differential geometric sense. This leads to the question for which projective variety Z, the Z-isotriviality of varieties of minimal rational tangents implies local flatness. Our main result verifies this for many cases of Z among complete intersections.Comment: Some errors in Section 8 and Lemma 8.1 corrected. To appear in The Asian Journal of Mathematics (AJM) special issue dedicated to Ngaiming Mok's 60th birthda

Engineering a serum-resistant and thermostable vesicular stomatitis virus G glycoprotein for pseudotyping retroviral and lentiviral vectors.

Vesicular stomatitis virus G glycoprotein (VSV-G) is the most widely used envelope protein for retroviral and lentiviral vector pseudotyping; however, serum inactivation of VSV-G pseudotyped vectors is a significant challenge for in vivo gene delivery. To address this problem, we conducted directed evolution of VSV-G to increase its resistance to human serum neutralization. After six selection cycles, numerous common mutations were present. On the basis of their location within VSV-G, we analyzed whether substitutions in several surface exposed residues could endow viral vectors with higher resistance to serum. S162T, T230N and T368A mutations enhanced serum resistance, and additionally K66T, T368A and E380K substitutions increased the thermostability of VSV-G pseudotyped retroviral vectors, an advantageous byproduct of the selection strategy. Analysis of a number of combined mutants revealed that VSV-G harboring T230N+T368A or K66T+S162T+T230N+T368A mutations exhibited both higher in vitro resistance to human serum and higher thermostability, as well as enhanced resistance to rabbit and mouse serum. Finally, lentiviral vectors pseudotyped with these variants were more resistant to human serum in a murine model. These serum-resistant and thermostable VSV-G variants may aid the application of retroviral and lentiviral vectors to gene therapy

Environmental Effects of Space Shuttle Solid Rocket Motor Exhaust Plumes

The deposition of NOx and HCl in the stratosphere from the space shuttle solid rocket motors (SRM) and exhaust plume is discussed. A detailed comparison between stratospheric deposition rates using the baseline SRM propellant and an alternate propellant, which replaces ammonium perchlorate by ammonium nitrate, shows the total NOx deposition rate to be approximately the same for each propellant. For both propellants the ratio of the deposition rates of NOx to total chlorine-containing species is negligibly small. Rocket exhaust ground cloud transport processes in the troposphere are also examined. A brief critique of the multilayer diffusion models (presently used for predicting pollutant deposition in the troposphere) is presented, and some detailed cloud rise calculations are compared with data for Titan 3C launches. The results show that, when launch time meteorological data are used as input, the model can reasonably predict measured cloud stabilization heights

Rocket exhaust ground cloud/atmospheric interactions

An attempt to identify and minimize the uncertainties and potential inaccuracies of the NASA Multilayer Diffusion Model (MDM) is performed using data from selected Titan 3 launches. The study is based on detailed parametric calculations using the MDM code and a comparative study of several other diffusion models, the NASA measurements, and the MDM. The results are discussed and evaluated. In addition, the physical/chemical processes taking place during the rocket cloud rise are analyzed. The exhaust properties and the deluge water effects are evaluated. A time-dependent model for two aerosol coagulations is developed and documented. Calculations using this model for dry deposition during cloud rise are made. A simple model for calculating physical properties such as temperature and air mass entrainment during cloud rise is also developed and incorporated with the aerosol model